Background: To evaluate the serum lipid profile in pre cancer subjects. Materials & Methods: A cohort comprising 20 participants was enlisted for the study, with their ages ranging from 45 to 65 years. Subsequent to the collection of laboratory samples, including blood, the levels of cholesterol, triglycerides, and HDL in the plasma were computed. The acquired data were then subjected to analysis using the SPSS software. Results: In Group II, referred to as the control group, there were also 10 patients, including 2 females and 8 male subjects. Within Group I, the triglyceride level was measured at 142.51 mg/dl, whereas in Group II, this level was noted as 198.7 mg/dl. The data revealed a statistically significant observation, with a P-value of less than 0.001. Conclusion: The change in lipid levels can have a diagnostic role in oral pre cancer subjects.