Background: Pancytopenia is characterized by a decreased number of at least two blood cell lines. The aim of this study was to evaluate the clinical presentation and etiological spectrum pancytopenias. Materials & methods: This was a prospective study conducted on 100 cases of age above 18 years who present to Dr DY Patil Medical College & Hospital and were found to have pancytopenia during hospital stay. Detailed history was taken. Relevant past and family history was taken. Findings of general and local examination were recorded wherever available. The results of routine investigations like CBC, RFT, LFT were recorded in all cases. The results of serum folate, serum B 12 level, ANA, Ultrasound Abdomen, Bone marrow aspiration and biopsy and other investigations done to reach to the diagnosis of pancytopenia were recorded. Results: Mean age of the patients was 41.84 years. 61 percent of the patients were males while the remaining were females. Pallor and generalized weakness was seen in 100 percent and 97 percent of the patients. Fever was present in 60 percent of the patients. Hepatomegaly, splenomegaly and lymphadenopathy were seen in 31 percent, 21 percent and 22 percent of the patients respectively. Megaloblastic anaemia was the most common etiologic factor found to be present in 63 percent of the patients. Dimorphic anaemia was found to be present in 12 percent of the patients while aplastic anaemia and sepsis were found to be present in 10 percent and 8 percent of patients respectively. Malaria and multiple myeloma were found to be present as etiologic factor in 5 percent and 2 percent of the patients respectively. Conclusion: Pancytopenia is not an uncommon hematological condition and is observed often in the clinical practice. Megaloblastic anemia was the major contributor to cause this condition followed by aplastic anemia. In terms of clinical presentations, the most common was pallor, followed by loss of weight, dyspnoea, fever. Other contributors were hepatomegaly, splenomegaly and weakness. Key words: Pancytopenia, Profile