Background: The present study assessed calcium and pH release in newer calcium silicate based root canal sealants. Materials & Methods: The present study was conducted in the department of Endodontics. Tubes were divided into 3 groups. Group I were control tubes, group II had MTA, group III had biodentine. In all tubes pH and calcium ions release was assessed at 24 hours, 7 days and 28 days. Results: In group I at 24 hours, pH was 6.94, in group II was 8.24 and in group III was 8.26. At 7 days it was 6.84, 8.37 and 8.60 respectively. At 28 days, it was 6.60, 8.32 and 8.76 in group I, II and III respectively. The difference was significant (P< 0.05). The mean calcium ion release (ppm) at 24 hours, 7 days and 28 days in group I was 0.06, 0.07 and 0.07 in group I, 9.06, 8.26 and 10.42 in group II and 5.72, 10.8 and 18.27 in group III. There was significant difference in all groups (P< 0.05). Conclusion: Authors found that biodentine showed significantly higher pH and calcium ion release than both MTA and control. Key words: Biodentine, MTA, Root canal filling