Background: The present study was conducted to assess post operative pain in single sitting root canal treatment. Materials & Methods: The present study was conducted on 60 single rooted teeth. Teeth were treated in single visit the root canal therapy. Biomechanical preparation was done followed by obturation by gutta percha. Post operative pain was evaluated using VAS scale after 6 hours, 12 hours, 12 hours, 24 hours and 48 hours. Results: Out of 45 patients, males were 25 and females were 20. The mean VAS after 6 hours was 8.2, at 12 hours was 5.4, at 24 hours was 3.6 and at 48 hours was 2.1. The difference was significant (P< 0.05). Conclusion: Authors found that there was significant reduction in post operative pain following single sitting root canal treatment. Key words: Root canal treatment, Single sitting, VAS.