Background: The root canal was packed with this non-plastising gutta-percha in cold lateral compaction, which was gradually moved towards a thermoplastising rubber-like material aimed at increasing root canal adaptability. The cold lateral condensation technique is the most frequently used obturation techniques by general dentists, and it is used in many countries. The present study was undertaken for assessing the efficacy of three different gutta-percha obturation techniques during root canal therapy. Materials & methods:75 extracted single rooted teeth (mandibular first premolars) were obtained and were stored in Saline.Three groups were formed: Group A – 25 single rooted teeth were obturated with Lateral condensation, Group B – 25 single rooted teeth were obturated with Single cone technique, and Group C – 25 single rooted teeth were obturated with Obtura II technique. The extent of Dye penetration was measured using Stereo microscope of 20x magnification. Measurements were recorded from the apex of the tooth to the most coronal extent of the dye penetration. All the results were recorded in Microsoft excel sheet and were analysed by SPSS. Results: Mean microleakage among specimens of group 1, group 2 and group 3 was found to be 0.25 mm, 0.24 mm and 0.28 mm respectively. While making inter-group comparison, non-significant results were obtained. Conclusion: All the three obturation techniques can be used with equal effectiveness in patients undergoing root canal therapy. Key words: Gutta-Percha, Obturation