Background: The present study was undertaken for assessing the effectiveness of manual and ultrasonic technique for removal of Calcium Hydroxide medicament from root canals. Materials and methods: A total of 100 freshly extracted maxillary second premolars were included. Carious, grossly decayed and deformed tooth specimens were excluded. All the specimens were stored in normal saline before use. Access opening was done and biomechanical preparation was done in all the specimens. Root canal medicament (CH) was applied in all the specimens. After application all the specimens were divided into two study groups as follows: Group A: CH was removed by manual technique. Group B: CH was removed by ultrasonic technique. After, removal of calcium hydroxide, evaluation of empty canals was done by taking radiographs of roots. The statistical analysis of the data was done using SPSS software for windows. Results: Non-significant results were obtained while comparing the mean change in gray levels before and after CH removal in between specimens of both the study groups. Conclusion: Techniques are equally effective for removing calcium hydroxide from the root canals. Keywords: Manual, Ultrasonic, Calcium Hydroxide.