Background: The present study was undertaken for assessing the effectiveness of efficacy of resin based and Mineral trioxide aggregate based sealer against E. faecalis. Materials & Methods: The present study was conducted with the aim of assessing the efficacy of AH plus (resin based sealer) and Fillapex (MTA based sealer) against E. faecalis. For testing antibacterial potential of sealers by Agar diffusion test (ADT), E faecalis was used. Two study groups were formed as follows: Resin group: Resin based sealer (AH plus), and MTA group: MTA based Sealer. The diameter of the growth inhibition zones was measured. Results: Mean area of zone of inhibition among resin group at 24 hours, 48 hours and 72 hours was found to be 9.7, 7.8 and 7.5 respectively. Mean area of zone of inhibition among MTA group at 24 hours, 48 hours and 72 hours was found to be 9.1, 8.3 and 6.9 respectively. In the present study, non-significant results were obtained while making intergroup and intragroup comparison. Conclusion: Non-significant difference exists while comparing the effectiveness of AH plus and resin based sealer against E. Faecalis. Key words: E. faecalis, Mineral trioxide aggregate.