Background: In endodontically treated teeth, the root canal system is reinforced by obturating the root canal in order to increase the resistance of the tooth to compressive strength. To provide a hermetic seal, the bonding of root canal sealer to the dentine is paramount in maintaining the integrity of the seal in a root canal filling. Hence; the present study was undertaken for assessing and comparing the efficacy of two different Root canal sealers used on the fracture resistance of endodontically treated teeth. Materials & methods: A total of 50 freshly extracted maxillary first premolars were included in the present study. All the specimens were divided into two study groups with 25 specimens in each group as follows: Group A: AH plus sealer was used, and Group B: MTA Fillapex sealer was used. After completion of biomechanical preparation, obturation was done according to the respective groups. After completion of root canal therapy, all the specimens were cut from apical one-third and were subjected to universal force testing machine for evaluating the fracture resistance. Results: Mean fracture resistance among specimens of group A and group B was found to be 253.4 N and 166.4 N respectively. In the present study, while doing statistical analysis, it was seen that mean fracture resistance among specimens of group A was significantly higher in comparison to specimens of group B. Conclusion: Fracture resistance of AH plus sealer is significantly higher in comparison to MTA Fillapex sealer. Key words: Root canal sealers, Fracture resistance