Background: Different types of chemical solutions have been recommended for denture disinfection. An ideal denture cleanser should be biocompatible, bactericidal, fungicidal, harmless, and nontoxic to the structure of denture; should be effectively remove deposits; and should be easy to use. Material and method: 90 heat cure acrylic samples were incorporated in this study. All samples had uniform dimensions and shape (50mm×30mm×3mm). These samples were divided into 3 groups based on the material to be used for immersion. The first group of samples were immersed in distilled water. The second and third group of samples were immersed in denture cleaner and thyme essential oil respectively. A 3-pointbending test till fracture was used to evaluate the fracture strength with the help of universal testing machine. Results: Control group recorded a mean value of 80.2 Mpa. The mean values of group 2 and group 3 were 87.6 and 92.4 Mpa respectively. The one way ANOVA analysis data showed statistically significant decrease with a P-value of .03. Conclusion: Thyme essential oil showed better flexural strength of acrylic resin than immersed in denture cleanser and control group. Key words: Thyme essential oil, heat cure acrylic resin, denture cleaner.