Background: Elastomeric impression materials offer high elastic recovery and acceptable flexibility on removal of the impression from the mouth. The present study was conducted to assess the effect of autoclaving on dimensional stability of elastomeric impression material. Materials & Methods: Standardized stainless steel die was prepared. Polyvinyl siloxane (Affinis) light body and putty viscosity elastomeric impression materials were used. A total of 30 impressions of the stainless steel die were made. Distance between the cross lines CD and C0 D0 reproduced in the impression were measured before autoclaving, immediately after autoclaving and 24 hours after autoclaving and dimensional change was calculated. Results: The mean value of dimensional change was 3.92 before autoclaving, 4.06 immediate after autoclaving and 3.57 after 24 hours. The difference of dimensional change at different intervals found to be non- significant (P> 0.05). Conclusion: Authors found that autoclaving does not cause significant dimensional change of elastomeric impression material. Key words: Autoclaving, Elastomeric impression material, Dimensional change