Background: Orthodontic tooth movement (OTM) is a biologic event which is facilitated by remodeling of periodontal ligament (PDL) and alveolar bone in response to the applied mechanical stimuli. Hence; under the light of above mentioned data, the present study was undertaken for assessing the effect of orthodontic treatment on dental pulp. Materials & methods: A total of 50 patients who underwent orthodontic treatment during the study period were included in the present study. Complete demographic details of all the patients were obtained. Radiographs were taken during the pre-treatment phase and findings were recorded in separate Performa. CBCT was done both during pre-treatment phase and post-treatment phase. Prevalence of pre-treatment and post-treatment pulp stones was assessed using the radiological findings. Also, on doing CBCT analysis, mean volume of pulp tissue was assessed both during pre-treatment and post-treatment phase and were evaluated. Results: Incidence of pulp stones during the pre-treatment and post-treatment phase was found to be 24 percent and 38 percent respectively. Significant results were obtained while comparing the incidence pre-treatment and post-treatment pulp stones. In the present study, mean pre-treatment pulp volume of incisors was found to be 46.36 mm3 while mean post-treatment pulp volume of incisors was found to be 42.89 mm3 respectively. Mean pre-treatment pulp volume of canines was found to be 52.12 mm3 while mean post-treatment pulp volume of canines was found to be 49.36 mm3 respectively. While comparing the pre-treatment and post-treatment pulp volumes among incisors and canines, significant results were obtained. Conclusion: Dental pulp tissue is affected significantly under the effect of orthodontic forces. Key words: Orthodontic treatment, Dental pulp.