Background: Denture hygiene is of utmost importance because dentures are used by the patients throughout the day and are in constant touch with oral environment including various microorganisms. Chemical cleansers are alternatives to mechanical cleaning. For cleaning, dentures should be immersed in the chemical solutions for a certain period of time. These solutions may include one or a combination of various active agents, such as sodium hypochlorite (NaOCl), chlorhexidine, alkaline peroxides, enzymes, and diluted acids. Aim of the study: To evaluate effects of denture cleansers on surface roughness of heat cure acrylic dentures. Materials and methods: The present study was conducted in the Department of Prosthodontics of the dental institutions. Total of 20 specimens of heat cure denture base resin were fabricated for evaluation of effect of various denture cleaners on surface roughness. Disc shaped wax patterns (10 mm in diameter and 2 mm thick) for surface roughness testing were fabricated using stainless steel mould of desired dimension and were invested in Type III gypsum product in a metallic flask. Results: We observed that difference in surface roughness was 0.22 µm with NaOCl, 0.09 µm with vinegar and 0.01 µm with control solution. Highest mean difference in surface roughness was seen with NaOCl and minimum with control solution. Conclusion: Within the limitations of the present study, it can be concluded that sodium hypochlorite resulted in increase in surface roughness as compared to Vinegar. Thus, NaOCl can be detrimental to prosthesis when used for longer duration. Keywords: Denture, Denture cleaners, Surface roughness