Background: Dental pulp is an unmineralized living tissue survival of which depends on the continuous blood flow. Some factors that have been implicated in pulp stone formation include age, impaired pulpal blood supply, genetic predisposition, or long-standing irritants such as caries, deep fillings, or abrasion. Hence; the present study was conducted for assessing the effect of orthodontic treatment on pulp stone formation. Materials & methods: A total of 50 patients were enrolled in the present study. Complete demographic details of all the patients were obtained. Clinical examination of all the patients was carried out. Panoramic radiographic, intraoral Periapical radiographs of all the patients was obtained both before the starting of the orthodontic treatment and after finishing of the orthodontic treatment. Incidence of pulp stones both before the starting of the orthodontic treatment and after finishing of the orthodontic treatment was recorded. Results: Pulp stones were found to be present in 6 percent of the patients during the pre-treatment phase, while it was present in 16 percent of the patients post-treatment. Conclusion: Orthodontic treatment might induce the formation of pulp stones. Key words: Orthodontic treatment, Pulp stones