Background: Cancer and precancerous lesions of the oral cavity is the most common neoplasm in the developing countries. Hence; the present study was undertaken for assessing the serum zinc levels in oral leukoplakia patients. Materials & Methods: A total of 50 patients who reported with a white patch and were histopathological proven oral leukoplakia were enrolled in the present study. Also 50 healthy subjects with negative oral findings and negative tobacco history of any other deleterious habit history were also included in the present study. Complete demographic details of all the subjects were obtained. The patients with oral leukoplakia were categorized as Group A while the healthy subjects were categorized as Group B. Serum samples were obtained from all the patients in sterile tubes and were transported to laboratory under aseptic conditions. In the laboratory, an auto-analyzer was used for assessment of serum zinc levels. All the results thus obtained were subjected to statistical analyses and were evaluated by SPSS software. Results: Mean serum zinc levels among the patients of the Group A and group B was found to be 78.13 µg/dL and 110.38 µg/dL respectively. A significant reduction in the mean serum zinc levels among the patients of the oral leukoplakia group was observed in comparison to the healthy controls. Conclusion: Zinc has a definitive role in the transition from normal mucosal to premalignant state. However; further studies are recommended. Key words: Oral leukoplakia, Zinc