Background: When a tooth has signs and symptoms indicative of irreversible pulpitis and periapical lesion, root canal treatment has traditionally been recommended. This present study was conducted to compare MTA and Biodentine in teeth with irreversible pulpitis. Materials & Methods: The present study was conducted on 30 mandibular premolar teeth of both genders. In all patients, endodontic procedure was performed by single endodontist. Teeth were divided into 2 groups. Group 1 patients were treated with MTA and group II with biodentine. Pain intensity was recorded after 24 hours, 1 week, 3 months and 6 months. Clinical assessment was done at 3 months and 6 months. Results: Pain score after 24 hours in group I was 1.8, in group II was 1.5, after 1 week was 0.59 and in group II was 0.68, after 3 months was 0.61 in group I and 0.60 in group II and 0.41 in group I and 0.46 in group II after 6 months. The difference was non- significant (P> 0.05). Clinical success rate after 3 months in group I was 82.1%, in group II was 81.8% and in group II was 83.2% at 6 months. The difference was non- significant (P> 0.05). Conclusion: Authors found that mineral trioxide aggregate and Biodentine were equally effective as pulp capping agents. Key words: Biodentine, Mineral trioxide aggregate, Pulp Capping