Background: Post-operative pain is a common finding after endodontic treatment. The present study was conducted to assess role of triple antibiotic paste (TAP) as an intra-canal medication in reducing post-operative pain. Materials & Methods: The present study was conducted on 46 patients of both genders. In all patients, intracanal medicament was sued and post-operative pain was assessed. Results: Out of 46 patients, males were 26 and females were 20. Post- operative VAS was 3.4 on 1st day, 2.1 in 3rd day and 1.4 on 7th day. The difference was significant (P< 0.05). Conclusion: Authors found that triple antibiotic paste is an effective intracanal medicament which can be used to relieve post operative pain. Key words: Pain, Triple antibiotic paste, Intra-canal