Objectives: To compare Knowledge about Oral health, hygiene and practices among under-graduate and post-graduate dental students in a Health care center at Jamshedpur, Jharkhand, India. Materials and methods: Four hundred thirty BDS and sixty MDS students from Awadh Dental College and Hospital of Kolhan University, Jamshedpur, Jharkhand respectively were invited to participate in this survey and they were asked to answer 13 questions, which were meant to evaluate the Knowledge about Oral health, hygiene and practice. The obtained data was analyzed using the Statistical Package for the Social Sciences version 20 software. Results: On comparison of the scores of knowledge, attitude, and practice, the mean knowledge score was significantly higher among post-graduates, final years and interns in compare to students of first to third year. The study also showed that female students had better oral health knowledge and showed better oral health practices than male students.Although senior and femalestudents had better knowledge and attitude towards oral health, there was a lack of adequate practice among them. Conclusion: Further emphasis on oral health is necessary in undergraduate and postgraduate training to improve oral health knowledge, attitude, and practice among dental students as they will act as role models for oral health education among individuals and community at large. Key words: Oral hygiene; dental students; Attitude; knowledge; practice