Background: Orthodontic devices exhibit a high degree of biocompatibility; however, there are reports of side effects linked to the release of nickel ions. Hence; the present study was conducted for assessing the effect of fixed orthodontic treatment on salivary Nickel levels. Materials & methods: Twenty patients receiving fixed orthodontic treatment were enrolled in the study. The orthodontic wires utilized in a sequential fashion were composed of nickel-titanium. Sample collection involved rinsing the mouth with 15 mL of distilled and deionized water for 30 seconds. Approximately 5 mL of saliva was obtained from each participant by expectoration into a beaker, which was then transferred to a designated polypropylene container. An auto-analyzer was employed to measure the ion concentration in the saliva samples, with nickel ion concentrations recorded in parts per billion (ppb). Statistical analysis was subsequently conducted. Results: A total of 20 patients were enrolled. Mean age of the patients was 18.6 years. Majority proportion of patients were males. Mean salivary nickel levels at pre-treatment, post-alignment and 6 months post-treatment was 42.3 ppb, 60.9 ppb and 49.8 ppb respectively. Significant results were obtained while comparing the mean salivary nickel levels at different time intervals. Conclusion: The concentration of nickel ions in saliva, despite being below the recommended daily allowance, warrants attention due to emerging insights into their molecular effects and potential for inducing allergic reactions. Key words: Nickel, Orthodontic, Fixed