Background: Gallstone disease is a chronic and recurrent condition affecting the hepatobiliary system. The metabolic processes of lipids and bile acids are interconnected. Despite this functional relationship, the impact of gallbladder removal on lipid metabolism remains inadequately understood. Therefore, this study was undertaken to evaluate the lipid profiles of patients undergoing laparoscopic cholecystectomy. Materials & methods: A total of 50 patients presenting with the primary complaint of cholelithiasis and scheduled for laparoscopic cholecystectomy (LC) were included in the study. Each patient received a comprehensive explanation of the surgical procedure prior to the operation. Blood samples were collected from all participants before the initiation of the surgery. Following the completion of the laparoscopic cholecystectomy, additional blood samples were obtained for comparison with the preoperative values. Results: A total of 50 patients participated in the study, with an average age of 45.3 years. The majority of the participants were female. In the preoperative phase, the mean levels of total cholesterol (TC), high-density lipoprotein (HDL), and triglycerides (TG) were recorded at 169.4 mg/dL, 49.5 mg/dL, and 193.7 mg/dL, respectively. Postoperatively, the mean levels of TC, HDL, and TG were observed to be 147.6 mg/dL, 41.1 mg/dL, and 210.9 mg/dL, respectively. Statistical analysis revealed a significant change in the mean lipid profile of all patients following the surgical intervention. Conclusion: Notable changes in the serum lipid profile are observed in individuals undergoing laparoscopic cholecystectomy.