Background: This study was conducted for comparing the impact of desensitizing agents on the retention of crowns cemented with luting agents. Materials & methods: We chose 100 recently removed mandibular molar teeth. All of the teeth were kept in regular saline pending usage. To remove surface stains, each specimen underwent a thorough cleaning. The samples were then kept at room temperature in distilled water. Diamond point was used to cut notches on the surface of the roots. A metal mould containing auto-polymerizing acrylic resin was partially filled with all of the specimens. The specimens were kept in distilled water for storage. By creating a clamp that could hold a high-speed air-rotor hand piece, uniform taper was achieved. Two research cohorts were formed: Glass ionomer cement is the control group in Group A, while GC Tooth Mousse desensitizer is the study group in Group B. Results: The trial included 100 recently extracted molars, which were divided into two study groups: Group A, referred to as the "Control," received glass ionomer cement, while Group B, referred to as the "GC Tooth Mousse desensitizer," also received glass ionomer cement. The average tensile bond strength of specimens in group A was 61.8 kg, while specimens in group B had an average tensile strength of 59.7 kg. The statistical comparison yielded insignificant findings. Conclusion: Desensitising chemicals may be used while crowns are being made because they won't influence the luting cements' capacity for retention. Key words: Luting agent, Desensitizing agent, Crown