Muscles are an organ system, made of up fibres, that work together in coordinated systems to create movement. As a soft tissue system, there are three different types of muscles; skeletal, cardiac, and smooth muscles. The function of masticatory system is complex. The musculature is involved directly in several important phases of prosthodontic treatment. Most obvious is of course, the action of muscles as prime movers of the mandible and hence as the power of repeated occlusion of the teeth. In addition, they are also active during mastication, deglutition, and speech. They exert a direct and indirect influence upon the peripheral extension, shape and thickness of denture bases, the positions of teeth both horizontally and vertically, and facial appearance. Muscles are the primary focus in vertical dimension, the neutral zone, arch form, occlusal disease and even smile design. Hence, the article focuses on explaining the various aspects in which muscles play a role in prosthodontics. Keywords: Muscles, Prosthodontics