Background: To evaluate levels of alkaline phosphatase in gingival crevicular fluid in chronic periodontitis subjects with smoking. Materials & Methods: A group of 50 subjects participated in the study, with ages ranging from 30 to 70 years. All participants were smokers and had generalized chronic periodontitis. The collected data was subjected to statistical analysis, employing Student's paired t-test, and the results were evaluated using SPSS software. Results: Fifty patients were included in the study. Among smokers with chronic periodontitis, the average Gingival Index (GI) score was determined to be 1.482. This finding exhibited statistical significance with a p-value of less than 0.001. Conclusion: Serum ALP levels were high in smokers with chronic periodontitis. Keywords: Smoking, Chronic periodontitis, alkaline phosphatase.