Background: This study was conducted to assess salivary zincconcentrations in oral leukoplakia patients. Material and methods: In total, 100 people were recruited for this study. The subjects were separated into two groups: group I (control) and group II (leukoplakia). Inductively coupled mass spectrometry (ICP- MS) was used to measure zinc levels in the saliva of 50 patients with oral leukoplakia. The results were compared to 50 age and gender matched control volunteers. Results: In healthy individuals and patients with oral leukoplakia, the mean salivary Zn levels were 56.12 g/dL and 13.69 g/dL, respectively. Salivary Zn levels in the leukoplakia group were significantly lower (P = 0.001) than in the control group. Conclusions: Saliva can be utilized as a potential diagnostic technique to assess zinc levels in pre-malignant oral cavity lesions.