Background: The purpose of this study was to assess the concentrations of copper in saliva among individuals diagnosed with oral submucous fibrosis (OSMF). Material and methods: A total of 100 participants were enlisted for this study. The participants were separated into two groups: Group A, which served as the control group, and Group B, which consisted of individuals with oral submucous fibrosis (OSMF). Inductively coupled mass spectrometry (ICP-MS) was employed to assess the levels of copper in the saliva of 50 patients diagnosed with oral submucous fibrosis (OSMF). The data were compared with a control group consisting of 50 individuals who were matched in terms of age and sex. Results: A notable disparity was seen in the average salivary copper levels between individuals with oral submucous fibrosis (OSMF) and the control group. Subjects with oral submucous fibrosis (OSMF) exhibited elevated copper levels. Conclusions: Saliva has emerged as a promising diagnostic technique that holds potential for quickly assessing copper levels in precancerous lesions of the oral cavity.