Aim: To assess Nickel levels in the saliva of subjects undergoing fixed orthodontic treatment at 3 different time periods of treatment. Materials & Method:100 patients under the age of 30 who were scheduled to have fixed orthodontic treatment were included. Three samples of stimulated saliva were taken from each orthodontic patient at different time periods of orthodontic treatment i.e., before placing fixed appliance, 30 minutes after placing and 10 days afterwards in order to establish a baseline for salivary Ni levels. SPSS software was used to evaluate the outcomes. Comparison was evaluated using T-HSD test. Results: The patients were 24.3 years old on an average. Ni levels in saliva were 10 µg/L at the beginning of the study. After 30 minutes of placing the appliance, Ni levels were highest i.e., 32 µg/L. Following ten days of orthodontic therapy, there was a slight decrease in the measures, and the Ni levels were 25 µg/L. When comparing the Ni levels at various times, significant results were seen. Conclusion: After the installation of fixed orthodontic appliances, salivary Ni contents significantly rose in comparison to baseline values. Keywords: Orthodontic Treatment, Saliva, Nickel