Background: The present study was undertaken for assessing the effect of orthodontic treatment on dental pulp. Materials & methods: A total of 100 patients who underwent orthodontic treatment during the study period were included in the present study. Complete demographic details of all the patients were obtained. Radiographs were taken during the pre-treatment phase and findings were recorded in separate Performa. Mean volume of pulp tissue was assessed both during pre-treatment and post-treatment phase and were evaluated. Results: Significant results were obtained while comparing the incidence pre-treatment and post-treatment pulp stones. In the present study, mean pre-treatment pulp volume of incisors was found to be 42.3 mm3 while mean post-treatment pulp volume of incisors was found to be 38.1 mm3 respectively. Mean pre-treatment pulp volume of canines was found to be 54.6 mm3 while mean post-treatment pulp volume of canines was found to be 50.2 mm3 respectively. While comparing the pre-treatment and post-treatment pulp volumes among incisors and canines, significant results were obtained. Conclusion: Dental pulp tissue is affected significantly under the effect of orthodontic forces. Key words: Orthodontic treatment, Dental pulp.