Background: Successful root canal treatment is aimed for complete removal of microorganisms by meticulous chemomechanical preparation followed by three-dimensional sealing of the endodontic system. The present study was conducted to assessed antibacterial efficiency of Qmix in root canal system. Materials & Methods: 45 recently extracted mandibular teeth were sectioned upto the level of cervical margin and washed with the solution of sodium hypochlorite. Access opening and biomechanical preparation was performed. Samples were divided into 3 groups of 15 each. In group I, Qmix irrigating solution was used. In groups II, sodium hypochlorite irrigant was used and in group III no irrigation was done. Results: Group I had 8%, group II 22% and group III 92% colonies of E. Faecalis. The difference was significant (P< 0.05). Conclusion: Smear layer was efficiently removed and level of E. Faecalis was decreased with Qmix in comparison to sodium hypochlorite.