Background: Oral cancer is the sixth most common cancer worldwide and is highly prevalent in Indian population. One of tumor marker is serum β2-microglobulin (β2-m). Hence; the present study was undertaken for evaluating serum beta-2 microglobulin in oral cancer patients. Materials & Methods: A total of 30 patients with confirmed histopathologic diagnoses of oral squamous cell carcinoma (OSCC) and 30 healthy controls were enrolled. Complete demographic and clinical details of all the patients were obtained. Clinical examination of all the patients was carried out. Blood samples were obtained from all the patients and were sent to laboratory for assessment of serum beta-2 microglobulin levels. All the results were recorded in Microsoft excel sheet and were analyzed by SPSS software. Results: Mean serum beta-2 microglobulin levels among the patients of the OSCC group and control group was 2.96 mg/L and 1.28 mg/L respectively. While comparing statistically, significant results were obtained. Conclusion: The estimation of serum β2-microglobulin may be useful as one of a battery of tests in the assessment of oral carcinoma patients. Key words: Beta-2 microglobulin, Oral cancer