Background: Our understanding of the causes, diagnosis, and treatment of acute myocardial infarction (AMI) has evolved significantly over the last 40 years. The present study was conducted for assessing the serum potassium levels in AMI patients. Materials & methods: A total of 25 AMI patients and 25 healthy controls were enrolled. AMI patients were regarded as study group and healthy subjects were enrolled as control group. All the patients were recalled in morning and blood samples were obtained. All the samples were sent to laboratory where auto-analyzer was used for evaluation of serum potassium levels. All the results were recorded and analyzed using SPSS software. Results: Mean serum potassium levels were higher in the control group (4.49 mEq/L) in comparison to the study group (3.82 mEq/L), the values of which were found to be statistically significant (P- value < 0.05). Conclusion: Serum electrolyte levels are altered significantly in AMI patients. Key words: Potassium, Myocardial infarction