Background: Disinfection of the root canal system is a specific requirement for endodontic treatment success. The present study was conducted to compare ultrasonic irrigation and syringe irrigation in clinical and laboratories study. Materials & Methods: 2 groups of 30 teeth each was made. In group I, ultrasonic irrigation and in group II, syringe irrigation was performed. The incidence of pain, quality of root canal filling and the number of obturated lateral canals were recorded. Results: The mean irrigation time in group I was 130.4 seconds and in group II was 216.2 seconds. There were 6 cases in group I and 3 cases of incidence of pain in group II. The difference was significant (P< 0.05). In group I, appropriate filling was seen in 24 and under filling in 6 cases and in group II, appropriate filling was seen in 23, over filling in 3 and under filling in 4 cases. The difference was significant (P< 0.05). Conclusion: Ultrasonic irrigation was effective in cleaning instrumented root canals than syringe irrigation. Key words: Syringe irrigation, Root canal treatment, Ultrasonic irrigation