Aim: This retrospective study was aimed to study different histopathological patterns as diagnosed in various gall bladder lesions. Materials & Methods: This study was conducted during 2019 (Jan-Oct) in the department of pathology, career institute of medical science and hospital, Lucknow. Total 200 specimen of cholecystectomy were studied for histopathological changes and diagnosis. Study slides were prepared by routine methods utilizing standard protocols. This study included non neoplastic and neoplastic gall bladder lesions. Acalculous and calculous gall bladder both type of specimen were included in study. Statistical Analysis and Results: Statistical analysis was done by statistical software Statistical Package for the Social Sciences (SPSS). The resultant data was sent to suitable statistical tests to achieve p values, mean, standard deviation, standard error an 95% CI. P ≤ 0.05 was considered as statistically significant. All patients were further separated into three age groups. 20 specimens were noted with single stone while 120 specimens were identified with multiple stones. Total 60 specimens did not show any stone in their examinations. Maximum 85 specimens were confirmed for Chronic Cholecystitis with Cholelithiasis with multiple stones followed by Acalculous Chronic Cholecystitis. Conclusion: Histopathological assessments confirmed that non neoplastic lesions are common in which chronic cholecystitis with cholelithiasis is commonest lesion. Moreover, neoplastic lesions are uncommon lesion in which adenocarcinoma are rare lesion. These data was only limited to the studied specimens of a particular territory therefore, author recommend other studies to be conducted on larger sample and on wider geographical area. Key words: Cholecystitis, Histopathology, Gall Bladder, Cholecystectomy