Background: The process of bone loss starts at menopause which is 2-5% per year due to declining estrogens levels and is seen in the first 5-7 years after menopause. The present study was conducted to assess serum calcium level in post-menopausal women. Materials & Methods: 104 post-menopausal women (Group I) and equal number of pre- menopausal women was taken (Group II). A thorough clinical examination was done. 3–5 ml of venous blood was drawn aseptically from antecubital vein of each subject. Serum calcium level was estimated. Results: Age group 45-50 years had 15, 50-55 years had 23, 55-60 years had 25, 60-65 years had 20 and >65 years had 17 subjects. The difference was non- significant (P> 0.05). The mean serum calcium level in group I was 8.5 mg/dl and in group II was 7.2 mg/dl. The difference was significant (P< 0.05). Conclusion: Post-menopausal women exhibited significantly lower serum calcium level as compared to pre- menopausal women