Background: Myopia is characterized by blurring of objects viewed at a distance, and is commonly the result of abnormal elongation of the eyeball – which causes the refractive image formed by the cornea and the lens to fall in front of the photoreceptors of the retina. Imaging modalities such as optical coherence tomography (OCT) can aid in the diagnostic dilemma by measuring retinal nerve fiber layer (RNFL) thickness, which differs significantly between glaucoma patients and controls. Hence; the present study was undertaken for assessing RNFL thickness in myopic patients using Spectral Domain OCT (SD-OCT). Materials & methods: A total of 20 high myopic patients and 20 low myopic patients were enrolled in the present study. Complete demographic and clinical details of all the patients were recorded. All patients were subjected to a comprehensive ocular examination. Visual acuity was measured using Snellen chart, read at a distance of 6m. Axial length and anterior chamber depth was measured. OCT- RNFL was done to measure retinal nerve fibre layer thickness. The data was collected and entered into the patient proforma. SPSS software was used for assessment of level of significance. Results: Mean RNFL average thickness among the patients with high myopia and low myopia group was 88.45 and 99.58 respectively. Significant results were obtained while comparing the mean RNFL average thickness among the patients of the severe myopia group and low myopia group. Conclusion: From the above results, the authors concluded that mean RNFL thickness is significantly reduced among patients with increasing grades of myopia.