Background: This study was aimed to investigate the influence of mandibular third molar position on lower anterior crowding in adult patients. Methodology: A sample of 70 patients (45 males and 25 females) were divided on the basis of lower third molar position into three groups: erupted group, unerupted group, and agenesis or absent group. Orthopantomograms and dental casts of all 70 samples were obtained for the study. Modified arch analysis proposed by Lundstorm was used for individual quantitative analytical registration of crowding. Results: There was no statistically significant difference in lower anterior crowding between the groups with erupted, unerupted, and agenesis of third molars as revealed by Chi-square test and ANOVA statistical tests. Conclusion: The mandibular third molar position have no effect on the lower incisors crowding. There was no statistically significant difference in lower anterior crowding in patient with erupted third molars when compared to subjects with unerupted, and absent third molars. The recommendation to extract third molars in the lower jaw cannot be solely done on the basis of doubtful rationale to minimize future crowding of the lower anterior teeth. Keywords: Agenesis, third molar position, lower anterior crowding,