Background: If the Ca(OH)2 is not completely removed prior to obturation, then it has negative effect on root canal therapy success because it interferes with zinc oxide-eugenol (ZOE) based sealers and results in reduced setting time as well as crack in the sealer because it is loosened faster. Different irrigation techniques have been in use to determine this problem. Clinically, most commonly used technique for removal of (Ca[OH]2) medicament is master apical file combined with numerous irrigation solutions. Aim of the study: To compare manual and ultrasonic technique for removal of Calcium Hydroxide medicament from root canals. Materials and methods: The study was conducted in the Department of Conservative Dentistry and Endodontics of Dental Institution. For the study we selected 120 extracted maxillary central incisors. Teeth with incompletely formed apex and having morphological and structural anomalies were excluded from the study. The selected teeth were immersed in sodium hypochlorite solution for 3 days to remove any organic debris. After 30 days, teeth were removed from incubator and were divided into two groups, Group 1 and Group 2 based on the method of removal of CaOH2from the canal with 60 teeth in each group. In Group 1, the removal of CaOH2paste was done manually using Size 30 Flexo file and NaOCl as irrigant. In Group 2, the removal of CaOH2paste was done using ultrasonic instrumentation and NaOCl as irrigant. Results: We observed that on comparing the gray values before application and after application, there was a statistically significant difference which means after removal of CaOH2 from the canals, the canals did not attain its previous empty state (p<0.05). This difference was observed in both the techniques used. We observed that the difference was statistically non-significant between both the techniques. Conclusion: Within the limitations of the present study, it can be concluded that complete removal of calcium hydroxide was not achieved with both the techniques, however, both the techniques are fairly successful in removal of calcium hydroxide from the root canals. Keywords: Calcium hydroxide removal, root canal treatment, ultrasonic, NaOCl