Background: Orthodontic appliances are highly biocompatible, although some side effects associated with the release of nickel ions have been documented. Nickel has been systematically studied for detrimental effects at cell, tissue, organ, and organism levels. Hence; the present study was conducted for assessing the salivary nickel levels in patients undergoing fixed orthodontic treatment. Materials & methods: A total of 20 patients were enrolled. Complete demographic and clinical details of all the patients were obtained. Before the starting of the fixed orthodontic treatment, salivary samples were obtained from all the patients and salivary nickel levels were assessed using auto-analyzer. Similar samples were obtained from all the patients one month after starting of fixed orthodontic treatment and two months after starting of fixed orthodontic treatment. All the results obtained were tabulated and were subjected to statistical analysis. All the results were analyzed by SPSS software by employing student t test. Results: Mean salivary nickel levels during the pre-treatment time, One month after starting of treatment and Two months after starting of treatment was found to be 1.36 µg/L, 5.33 µg/L and 3.26 µg/L respectively. While analyzing statistically, it was observed that there was significant rise in serum nickel levels one month after starting of treatment followed by reduction after two months after starting of treatment. Conclusion: There is a transient rise in salivary nickel levels in patients undergoing fixed orthodontic treatment. Key words: Nickel, Orthodontic, Salivary