Background: Diabetes mellitus (DM) is a chronic condition characterized by chronic hyperglycemia that results from unregulated endocrine and metabolic pathways in glucose utilization. Periodontitis is a slowly progressing disease but the tissue destruction that occurs is largely irreversible. In the early stages, the condition is typically asymptomatic; it is not usually painful, and many patients are unaware until the condition has progressed enough to result in tooth mobility. Diabetic patients with chronic periodontitis have depressed chemotaxis compared with nondiabetic patients with chronic periodontitis. Aim of the study: To compare neutrophil functions in diabetic and healthy subjects with chronic generalized periodontitis. Materials and methods: The present study was conducted in the Department Periodontics of the dental institution. A total of 40 subjects were selected for the study. 20 were diabetics and 20 were non diabetics with chronic periodontitis. Patient included in the study had 20 teeth present and had >2 mm of attachment loss at periodontitis sites. The patients having any systemic disease other than diabetes were excluded from the study group and with history of any systemic disease were excluded from control group. The brief history of each patient including name, age, sex, past medical and dental history, plaque inde and gingival inde were recorded. Results: A total of 40 patients participated in this study. We observed that mean age in study group was 51.65 years and in control group was 53.24 years. Number of male participants in study group was 18 and female participants were 12. Similarly, number of male participants in control group was 16 and female participants were 14. We observed that mean neutrophil chemotaxis for study group was 20.35 and for control group was 33.65. The results on comparison were seen to be statistically significant (p<0.05). Conclusion: Within the limitations of the present study, it can be concluded that neutrophil activity is significantly reduced in diabetic patients. Keywords: Neutrophil, periodontitis, diabetes mellitus