Background: Periodontitis is characterized by periodontal bone loss, tooth mobility and ultimately exfoliation of teeth. Plaque accumulation around teeth is the reason for periodontal breakdown. The present study was conducted to assess the clinical and microbiological status of osseointegrated dental implant with that of a natural tooth. Materials & Methods: The present study was conducted in the department of Periodontics. It comprised of 20 patients (12 males and 8 females). Patients were divided into 2 groups. In group I patient, subgingival plaque sample was collected from subgingival site around implant and in group II subgingival plaque sample was collected from a subgingival site around natural tooth distal to implant. Parameters such as plaque index, sulcus bleeding Index probing pocket depth were recorded. Results: The mean probing depth in positive samples of porphyromonas gingivalis in group I was 4.38 and in group II was 3.65, sulcular bleeding index was 1.62 in group I and 1.03 in group II and plaque index was 2.10 in group I and 1.76 in group II. The difference was non- significant (P> 0.05). The mean probing depth in positive samples of prevotella intermedia in group I was 4.15 and in group II was 3.81, sulcular bleeding index was 1.48 in group I and 1.02 in group II and plaque index was 2.04 in group I and 1.71 in group II. The difference was non- significant (P> 0.05). The mean probing depth in positive samples of Tannerella forsythia in group I was 4.62 and in group II was 4.54, sulcular bleeding index was 2.06 in group I and 1.52 in group II and plaque index was 2.30 in group I and 2.18 in group II. The difference was non- significant (P> 0.05). Conclusion: Authors found that there was correlation between microorganisms such as Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and sulcular bleeding index, plaque index, and periodontal pockets. Key words: Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia.