Background: The color of teeth is strongly determined by dentin with more translucent enamel playing a lesser role through scattering at wavelengths in the blue range. The present study was conducted to assess tooth color, eye color and skin color and its relation with gender. Materials & Methods: The present study was conducted on 120 subjects of age ranged 20-40 years of both genders. Eye color or iris color was visually inspected and was assessed as: score 1 was for Blue, blue/green light, score 2 was for brown/ gray, score 3 was for dark brown and score 4 was for black. Skin color was determined on the forehead at 3 cm above the nasal bridge and then it was categorized as: score 1 for very light, 2 for light, 3 for middle and 4 for dark. Tooth color was measured using Vita Easy Shade Advance and expressed as L, C and H. Results: Age group 20-30 years had 35 males and 32 females and 30-40 years had 25 males and 28 females. A negative and significant correlation between skin color and L* in males and females was found. A negative and significant correlation was seen between eye color and chroma of tooth (P< 0.05). A negative but significant correlation between age and L* in males and females (P< 0.05) was observed. Conclusion: Authors found that hue, value and chroma of tooth are partially dependent on skin color, eye color, age, and gender. Key words: Chroma, hue, value.