Background: In orthodontics, the various components of fixed appliances are fabricated by the use of varying materials which have their own physical and mechanical properties. Nickel and chromium ions released from fixed orthodontic appliances can serve as allergens or may have serious biological side effects. Hence; the present study was conducted for assessing salivary nickel levels in patients undergoing orthodontic treatment. Materials & methods: A total of 20 patients were enrolled in the present study. Complete demographic details of all the patients were obtained. Clinical examination was carried out with mouth mirror and probe. Salivary samples were obtained and were sent to laboratory. Orthodontic brackets were placed and orthodontic treatment was started and salivary samples were obtained at 1 week after placement of orthodontic appliance and 1 month after placement of orthodontic appliance. In the laboratory, auto-analyzer was used for evaluation of salivary nickel levels. All the results were recorded in Microsoft excel sheet and were analyzed by SPSS software. Results: Mean salivary nickel levels before placement of appliance, 1 week after placement of appliance, and 1 month after placement of appliance were found to be 1.214µg/L, 7.897µg/L and 4.196µg/L respectively. While comparing statistically, it was observed that a significant rise in the salivary nickel levels occur one week after placement of orthodontic appliance followed by a significant fall in its concentration 1 month afterplacement of orthodontic appliance. Conclusion: Salivary nickel levels are raised significantly during the course of orthodontic treatment. Key words: Nickel, Orthodontic, Salivary