Aim: The aim of this study is to assess Saudi mothers’ knowledge and awareness of preschoolers’ oral hygiene and the importance of their first dental visit. Materials and Methods: A cross-sectional survey was conducted in Riyadh Elm University hospitals, Riyadh, Kingdom of Saudi Arabia. An online questionnaire was distributed to all mothers of children aged 6 months to 3 years. Data were analyzed using SPSS Version 23. Descriptive statistics were performed to present the overview of the findings. Results: A total of 639 participants responded to the survey. There was no statistically significant association between knowledge and awareness with demographic characteristics. Only 39.6% reported that the first dental visit for their child should be at 1 year. Conclusions: In conclusion, Saudi mothers in Riyadh city regardless of their educational level are in constant consciousness about children oral health situation and regarding the importance of primary teeth. Key words: Mother, Knowledge, Awareness, Preschooler, Oral hygiene