Introduction: Fiber posts associated with resin cores are used in structurally compromised teeth to retain the final restoration. The residual root canal sealers left on dentin walls after post space preparation might affect the adhesion between the fiber posts and root dentin. The aim of this study was to evaluate the effects of calcium silicate sealer (Endosequence BC sealer), epoxy resin sealer (AH Plus) and zinc oxide eugenol based sealer (Tubliseal) on bond strength of fiber post to root dentin. Methods: Sixty extracted human maxillary incisors were decoronated and prepared using step back technique upto ISO #40. The samples were divided into 3 experimental groups and one control group depending upon the type of sealer used. In control group A, no sealer was used for the obturation. In groups B, C and D, the canals were filled with gutta-percha using Tubliseal, AH Plus and Endosequence BC sealers, respectively, by cold lateral compaction technique. After post space preparation, the fiber posts were cemented in the root canals using self-etch adhesive Rely X U200. Then 1-mm-thick disks were prepared from the coronal thirds of all the root canals and subjected to a push-out bond test using a universal testing machine. Mode of failure was determined using a stereomicroscope. Data were analyzed using the one-way ANOVA and post hoc Tukey’s tests. Results: The control group demonstrated maximum bond strength and the Tubliseal showed least bond strength. The push-out bond strength values were similar for Endosequence BC sealer and AH Plus sealer (P>0.05). These values were significantly higher than that of the Tubliseal sealer (P<0.05). Conclusion: All the sealers tested decreased the bond strength of the fiber posts, with Tubliseal showing the greatest reduction in the bond strength