Background: Root perforation is an artificial communication between the root canal system to the supporting tissues of the tooth or to the oral cavity. Despite the favorable properties of MTA that supports its clinical use, it has several clinical drawbacks such as prolonged setting time, difficult handling characteristics, and potential discoloration. Biodentineis a high purity calcium silicate based dental material composed of tricalcium silicate; calcium carbonate, zirconium oxide, and water-based liquid containing calcium chloride as the setting accelerator and water-reducing agent.Hence; we planned the present study to evaluate the sealing ability of white Mineral trioxide aggregate, Biodentine, Calcium phosphate cement, and Glass ionomer cement as furcation repair materials using dye extraction leakage method. Materials & methods: Forty-five extracted, intact human mandibular molars with non-fused and well-developed roots were used in this study. Molars were decoronated 3 mm above the cemento-enamel junction and roots were amputated 3 mm below the furcation. Teeth were randomly divided into five groups with 10 samples each for the experimental group and 5 samples in the control group. Group I was repaired with white MTA (Angelus), Group II with Biodentine (Septodont, France), Group III with Glass Ionomer Cement (3M ESPE), Group IV with Calcium Phosphate Cement (GBone surgiwear) and Group V is control group which was left unrepaired (positive control). All the teeth in each group were allowed to set for 24 h. Each specimen was subjected to dye extraction and microleakage evaluation. This was followed by centrifugation and spectrophotometric evaluation.  The obtained readings were statistically analyzed using one-way analysis of variance and Tukey multiple comparisons tests. Results: There was a significant difference in the mean dye leakage among four groups (P < 0.001). There was a statistically significant difference seen between the White MTA and CPC groups, between Biodentine and GIC groups, also between Biodentine and CPC groups. As there are more values of absorbance in GIC, CPC, and control groups compared to White MTA and Biodentine groups. Conclusion: Among all four materials used viz. White MTA, Biodentine, Glass ionomer cement, and Calcium phosphate cement, Biodentine showed least dye absorbance and best sealing ability as compared to other materials due to differences in properties like specific surface area, pore volume and pore size.
Key words: Biodentine, Calcium phosphate cement, Glass ionomer cement, Sealing