Background: Oral submucous fibrosis (OSMF) is a chronic debilitating disease of the oral cavity.The pathogenesis for malignant transformation is not precisely known and is thought to be multifactorial. The carcinogenicity of tobacco in synergism with areca nut is well known, but recently, the carcinogenicity of areca nut alone without tobacco has also been identified. Of late, a renewed interest in the role of copper as a mediator of fibrosis in OSMF has been postulated. Hence; we planned the present study to evaluate the levels of copper in the blood serum of patients with OSMF and further compare them with the normal levels of copper. Materials & methods: The present study included in-vitro assessment of serum of copper levels in OSMF patients. A total of 20 cases of OSMF and 20 cases of without any lesion and habit (normal control) were included in the present study. Fasting blood samples (approx. 5ml) were collected and the serum obtained was kept in freeze at −20 C until it was analyzed for serum copper with atomic absorbtion spectrophotometry. All data analysis was carried out using Statistical Package for Social Science 17 for the descriptive analysis. Results: Significant results were obtained while comparing the mean serum copper levels among OSMF patients and normal controls. Conclusion: Serum copper levels could be used as potential prognostic and diagnostic markers in OSMF patients Key words: Copper, Oral Submucous fibrosis, Serum Corresponding Author: Dr. Vandana Katoch, Department of Oral and Maxillofacial Pathology, IDS, Sehora, Jammu, India. This article may be cited as: Katoch V, Singh N, Sidhu S, Saini S, Kumar M, Sirsalmath M. Estimation of Serum Copper Levels in Oral Submucous Fibrosis Patients: An in-vitro study. Int J Res Health Allied Sci 2017;3(6):95-97.