Background: Denture stomatitis affects almost 15 to 65% of healthy mouths who are denture wearers. The etiology of denture stomatitis is generally multifactorial which includes tissue trauma from ill fitting dentures, lack of denture cleanliness, dietary factors, continuous denture use without removal, chronic illness, and a compromised and poor immune system. Predisposing factors are inadequate denture hygiene, denture wearing habits, xerostomia, medications and nutritional factors. Aim: To compare the efficacy of Fluconazole and Nystatin incorporated into Tissue Conditioner as drug delivery method for denture stomatitis. Materials and method: The present study was conducted in the department of prosthodontics of the dental institution.  Candida albicans was cultured onto Sabouraud dextrose agar plate and incubated at 37ºC for 3 days.  All disks were contaminated with 100 μl of 1 × 10 6 CFU/ml C. albicans cell suspension and the cell culture plate were incubated at 35ºC on a rotary shaker for 48 hours. The plates were incubated at 37ºC for 48 hours and the colonies were enumerated. The data was tabulated for further evaluation. Results: Nystatin 5% solution was the most efficient for inhibiting attachment and colonization of C. albicans (0.22). Fluconazole 5% solution is partially effective efficient for inhibiting attachment and colonization of C. albicans. Conclusion: The incorporation of nystatin rather than fluconazole into tissue conditioners is more effective in treatment of chronic atrophic candidiasis in denture users. 
Keywords: Tissue conditioners, denture stomatitis, Candida, anti-fungal.

Corresponding Author: Dr. Mukesh Kumar, Professor, Department of Prosthodontics, Patna Dental College and Hospital, Patna, Bihar, India

This article may be cited as: Kumar M, Khajuria R, Sudan S, Sudan T, Choudhary P, Sodhi G. Comparative analysis of efficacy of Fluconazole and Nystatin incorporated into Tissue Conditioner as drug delivery method for Denture Stomatitis. Int J Res Health Allied Sci 2017;3(6):86-88.