Aim- The aim of this study was to assess changes in serum bilirubin levels before and after laparoscopic cholecystectomy (LC). Materials and methods- A total of 50 patients were included, and comprehensive demographic data were gathered for each. Blood samples were collected at baseline (preoperatively) and at 24 and 72 hours postoperatively to evaluate serum bilirubin levels. The changes in bilirubin were analyzed using SPSS software. Results- The mean total serum bilirubin levels were recorded at 0.45 mg/dL during the preoperative period, increasing to 1.12 mg/dL at 24 hours post-operation and decreasing to 0.47 mg/dL at 72 hours post-operation. Statistical analysis indicated significant differences among these values. Similarly, the mean serum direct bilirubin levels were found to be 0.36 mg/dL preoperatively, 0.63 mg/dL at 24 hours post-operation, and 0.38 mg/dL at 72 hours post-operation, with the comparisons also yielding statistically significant results. Conclusion- Significant alterations in serum bilirubin levels are observed in patients undergoing laparoscopic cholecystectomy. Keywords- laparoscopic, cholecystectomy, bilirubin