Background: This study was conducted to evaluate asymptomatic bacteriuria among diabetic females. Material and methods: 50 females between the ages of twenty-five and sixty-five years who met the American Diabetes Association's (ADA) definition of DM were enrolled in the study overall. Each subject was enrolled in the study after receiving written informed consent and approval from the institutional ethics committee. Patients with symptomatic UTIs, renal failure, obstructive uropathy, indwelling catheters, pregnant women, those with impaired immune systems, and people who had recently taken antibiotics were all excluded from the trial. HbA1C was performed on all individuals in addition to customary biochemical tests such complete blood count, blood urea, serum creatinine, fasting blood sugar, and viral indicators. All patients provided clean, midstream urine samples that were collected and processed in accordance with industry standards. Results: Overall, 50 females aged from 25–65 years, had been involved. Out of these, 36 females were found to be ASB positive, and 14 were ASB negative. The most frequent micro-organism isolated from ASB-positive patients was E. coli, taking into account for 19 out of 36 instances (52.7%), followed by Candida in 11 cases (30.5%), Pseudomonas aeruginosa (P. aeruginosa) in 3 (8.3%), 2 (5.5%) positive for Klebsiella pneumoniae (K. pneumoniae) and 1 (2.7%) positive for Citrobacter freundii (C. freundii). Conclusion: Patients with type 2 diabetes frequently experience ASB, and having poor glycemic control is a major risk factor for its occurrence. This demonstrates how crucial strict glycemic control is for preventing ASB. Keywords: bacteriuria, asymptomatic, diabetes.