Background: Vitamin D is produced in the skin through the assistance of ultraviolet-B radiation and serves multiple functions within the body. Temporomandibular disorders (TMDs) constitute a collection of pathological conditions that impact the temporomandibular joints, masticatory muscles, and adjacent tissues. Hence, this study was conducted to assess the vitamin d deficiency in subjects with temporomandibular disorders. Materials & Methods: A total of 40 individuals were enrolled in the study, evenly distributed into two categories: 20 participants diagnosed with TMDs and 20 control individuals. Among those diagnosed with TMDs, 12 were classified as female, while 8 were identified as male. The collected data underwent analysis using the SPSS software, and statistical significance was defined with P values below 0.05. Results: Forty patients were included in the study and categorized into two sections: the temporomandibular disorder (TMD) group and the control group, each comprising 20 participants. Conclusion: In TMDs, vitamin D deficiency should be assessed and corrected.