Aim: This study was undertaken to evaluate the percentage of gutta-percha-filled area (PGFA) using microscopic analysis of the cross-sections in the apical third of root canals when filled either with Thermafil technique, Warm Vertical Condensation technique and Cold Lateral Condensation technique without using sealers. Materials and methods: Forty five single rooted extracted permanent teeth were collected. After crown amputation, the teeth were randomly divided into three experimental groups of 15 specimens each. Group I—thermafil obturation technique, group II—warm vertical condensation obturation technique and group III—cold lateral condensation obturation technique. Obturation was performed by specific techniques without using sealers. After obturation, the teeth were cross-sectioned horizontally at 2 to 3 mm from apex with the help of double sided diamond disk. Sections were digitally photographed and measured under Stereomicroscope at magnification of 50×. Results: Maximum group difference was observed between groups I and III (3.558 ± 0.138) while minimum difference was observed between groups I and II (1.223 ± 0.137). Thus, all the between group differences were statistically significant. Conclusion: This study supports the belief that the Thermafil Obturation technique produces significantly higher percentage of gutta-percha filled area (PGFA) than the warm vertical condensation technique or cold lateral condensation technique. Keywords: Gutta-percha filled area, Thermafil, Warm vertical, Cold lateral condensation.