Background: Gallstones disease is a chronic recurrent hepatobiliary disease. Lipid and BA metabolisms are functionally interrelated. Even though lipid and BA metabolisms are functionally related, how gallbladder removal affects lipids is not well understood. Hence; the present study was conducted for assessing the lipid profile in patients undergoing LC. Materials & methods: Assessment of 20 patients reporting with chief complaint of Cholelithiasis and scheduled to undergo LC was enrolled. Entire surgical procedure was explained to all the patients. Blood samples were obtained from all the patients before the starting of the surgery. LC was carried out and blood samples were obtained again and compared with preoperative values. Results: A total of 20 patients were enrolled. Mean age of the patients was 45.3 years. Majority of the patients were females. During the preoperative period, mean TC levels, HDL levels and TG levels was found to be 172.3 mg %, 46.5 mg % and 183.6 mg % respectively. During the postoperative period, mean TC levels, HDL levels and TG levels was found to be 159.2 mg %, 44.8 mg % and 221.6 mg % respectively. While analysing statistically, significant alteration in the mean lipid profile of all the patients was seen postoperatively. Conclusion: Significant alterations in the serum lipid profile occur in patients undergoing laparoscopic cholecystectomy. Key word: Laparoscopic cholecystectomy, Lipid profile